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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: MicroRNA-133 overexpression promotes the therapeutic efficacy of mesenchymal stem cells on acute myocardial infarction
doi: 10.1186/s13287-017-0722-z
Figure Lengend Snippet: miR-133 enhanced survival of MSCs in vivo and downregulated expression of snail 1 . Survival of MSCs indicated by CM-Dil (chloromethylbenzamido) and expression level of snail 1 analyzed by Q-PCR. a Fluorescent photographs of surviving MSCs with red fluorescence. b miR-133 level in exosome isolated from MSC culture supernatant. c Western blot analysis of CD63 in exosome. d Snail 1 3′UTR contains predicted miR-133 binding sites, which are conserved among species. e Relative expression of snail 1 in cardiomyocytes cocultured with different MSCs. f Relative expression of snail 1 in left ventricular tissue at 7 and 28 days after MI. * p < 0.05, ** p < 0.01, *** p < 0.001. CON control, LV left ventricle, MSC mesenchymal stem cell, NC negative control, PBS phosphate-buffered saline
Article Snippet: To evaluate the effect of miR-133 on cell survival in vivo, normal MSCs, MSCs infected with empty lentivirus (vector-MSCs), or miR-133-overexpressed lentivirus (miR-133-MSCs) were dyed with
Techniques: In Vivo, Expressing, Fluorescence, Isolation, Western Blot, Binding Assay, Negative Control